Differentiation and Dedifferentiation of the Human Monocytic Leukemia Cell Line, U937.
نویسندگان
چکیده
منابع مشابه
Essential amino acid deprivation induces monocytic differentiation of the human HL-60 myeloid leukemia cell line.
In this study we examine the effects of amino acid deprivation on the growth and differentiation of the human HL-60 myeloid leukemia cell line. The HL-60 cell line was chosen for study because of its ability to differentiate along either a granulocytic or monocytic pathway under appropriate culture conditions. Differentiation was determined by changes in cell morphology, nonspecific esterase (N...
متن کاملCharacterization of the adhesion of the human monocytic cell line U937 to cultured endothelial cells.
Adhesion of blood-borne monocytes to the vascular endothelium is the first step in the infiltration of this leukocyte into the vessel wall or the interstitial space during inflammation. A significant role for the monocyte in both wound healing and atherogenesis is now well accepted. The molecular interactions involved in monocyte attachment to the endothelium are unknown. To study this phenomen...
متن کاملPurification of a 110-kilodalton cytosolic phospholipase A2 from the human monocytic cell line U937.
The major dithiothreitol-resistant phospholipase A2 activity present in the cytosol of U937 cells has been purified greater than 200,000-fold by sequential chromatography on phenyl-5PW, heparin-Sepharose CL-6B, high-performance hydroxylapatite, TSK-gel G3000-SW, and Mono Q columns. This 110-kDa cytosolic phospholipase A2 is distinct from the relatively small (14-kDa) dithiothreitol-sensitive ph...
متن کاملLymphokine inducing "terminal differentiation" of the human monoblast leukemia line U937: a role for gamma interferon.
The human monoblast leukemia line, U937, is growth-inhibited and induced to develop markers of mature monocytes by lymphokine preparations. Lymphokine is cytostatic and induces expression of Fc receptors in U937 and in myelomonocytic leukemic lines RC-2A and KG-1, but does not have these effects on T- and B-lymphocytic lines. In addition to previously described properties, including complement ...
متن کاملEffect of endogenous and exogenous interferons on the differentiation of human monocyte cell line U937.
The effect of interferons (IFNs) on the differentiation of hematopoietic cells was examined with the human monocyte cell line U937. The differentiation of U937 was induced by hydroxyvitamin D3 and was evaluated through the study of specific markers. The induction of the U937 differentiation was associated with a production of IFN and with a marked increase in (2'5') oligoadenylate synthetase. A...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell Structure and Function
سال: 1992
ISSN: 0386-7196,1347-3700
DOI: 10.1247/csf.17.263